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  • GE17-5181-01 - SOURCE 15Q 4.6/100 PE

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GE17-5181-01 Sigma-Aldrich

SOURCE 15Q 4.6/100 PE

  •  NACRES SB.52

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Properties

Related Categories Affinity Chromatography, Analytical/Chromatography, Anion Exchange Media - Ion Exchange Chromatography , GE Life Sciences Ion Exchange Columns, HPLC & FPLC Columns for Biopolymer Separations,
description   Ion Exchanger Type (value)
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 ea
parameter   <5 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  580 psi
bed size   4.6 mm × 100 mm
bed volume   1.7 mL
column I.D.   4.6 mm
matrix   polystyrene/divinylbenzene
matrix active group   ammonium ion, quaternary phase
avg. part. size   15 μm
particle size   15-15 μm
cleaning   1 - 14
working range   2 - 12
suitability   suitable for bioprocess medium
separation technique   anion exchange

Description

General description

SOURCE 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

Features and Benefits

• SOURCE 15Q media is highly suitable for fast, high-resolution purifications and easy scale-up
• Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
• Improved capacity compared to MonoBeads but with a slightly lower resolution.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

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Legal Information

SOURCE is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Appendix 10, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Buffers, Chromatography, Detergents, Ion Exchange, Separation, Solvents

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

Appendix 3, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Antimicrobials, Chromatography, Gene expression, Ion Exchange, Purification, Separation

Ion Exchange Chromatography Troubleshooting

If only certain peaks are of interest in this well-resolved separation, it may be advantageous to transfer to a step elution in order to save time and buffer. The rest of this section focuses on prac...
Keywords: Absorption, Adsorption, Buffers, Chromatography, Detergents, Digestions, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Solvents

Non-volatile and Volatile Buffer Systems

Appendix 2, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Chromatography, Ion Exchange

Practical Considerations for IEX Separation

This section covers detailed aspects of each step in an IEX separation, together with practical hints and tips to improve resolution and overall performance. In practice a separation can be summarize...
Keywords: Absorption, Buffers, Cell culture, Chromatography, Detergents, Filtration, Ion Exchange, Nucleic acid denaturation, PAGE, Phase transitions, Precipitation, Sample preparations, Separation, Solvents

SOURCE: Purification at High Throughput with High Resolution and Easy Scale-Up

Use SOURCE 15 for intermediate purification or polishing steps in laboratory or large-scale applications that require high resolution and high throughput (flows up to 1800 cm/h).
Keywords: Adsorption, Buffers, Chromatography, Detergents, High performance liquid chromatography, Ion Exchange, Precipitation, Purification, Sample preparations, Separation, Solvents

Sample Preparation in Ion Exchange Chromatography

Appendix 1, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Absorption, Adsorption, Affinity chromatography, Amplification, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Filtration, Indicators, Ion Exchange, Nucleic acid denaturation, PAGE, Precipitation, Purification, Sample preparations, Separation

Selection of Purification Equipment

Appendix 4 extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Chromatography, Ion Exchange, Purification, Separation

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