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GE17-5186-01 Sigma-Aldrich

SOURCE 15PHE 4.6/100 PE

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, GE SOURCE Column, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, Hydrophobic Interaction (HIC) Columns More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 ea
parameter   580 psi
bed size   4.6 mm × 100 mm
bed volume   1.7 mL
column I.D.   4.6 mm
matrix   polystyrene/divinylbenzene
avg. part. size   15 μm
particle size   15-15 μm
cleaning   1 - 14
working range   2 - 12
suitability   suitable for bioprocess medium
separation technique   hydrophobic interaction (HIC)

Description

General description

SOURCE 15PHE 4.6/100 PE is a Tricorn high performance column pre-packed with SOURCE 15PHE.

Application

SOURCE 15PHE 4.6/100 PE high capacity makes it an excellent choice for preparative hydrophobic interaction purifications at laboratory scale. In addition, its longer bed height provides increased resolution. The column is also useful for optimization studies during scale-up.

Features and Benefits

• High resolution separations at high loads and high fLow rates.
• Wide range of selectivities.
• Reproducible quality.
• Excellent scalability.
• High chemical and physical stability.
• Low backpressure.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

www.gelifesciences.com.

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Legal Information

SOURCE is a trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

Appendix 10, extracted from Ion Exchange Chromatography & Chromatofocusing, GE Healthcare, 2007
Keywords: Buffers, Chromatography, Detergents, Ion Exchange, Separation, Solvents

HIC Purification Options and Scale up with SOURCE™ Media

SOURCE™ media are based on a hydrophilic matrix made from monodispersed, rigid, polystyrene/divinyl benzene and substituted with hydrophobic ligands: phenyl, ether or isopropyl (Fig 29). The media de...
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange, Ligands, PAGE, Purification, Separation

Selection of Purifiation Equipment for Hydrophobic Interaction and Reversed Phase Chromatography

A chromatography system is required in order to achieve a high-resolution separation using accurately controlled linear gradient elution, to take advantage of the high flow rates of modern media, or ...
Keywords: Chromatography, Mass spectrometry, Separation

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Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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