GST Bulk Kit

Cytiva, 27-4570-01, pack of 1 kit

  •  NACRES NA.56



shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 kit
mfr. no.   Cytiva, 27-4570-01
shipped in   wet ice
storage temp.   2-8°C


General description

GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins.


GST Bulk Kit contains a 10 mL bulk pack of Glutathione Sepharose® 4B medium, five gravity-fLow columns, IPTG, and GST Buffer Kit. GST-tagged Proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of Glutathione Sepharose® 4B, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used.

Features and Benefits

• For rapid purification of GST-tagged Proteins using gravity flow columns or using batch purification with high specificity.
• Preserves Protein antigenicity and function.
• Yields up to 50 mg of pure Protein.

Analysis Note

Legal Information

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Preparative Purification Using GSTPrep FF 16/10 Column

Glutathione Sepharose 4B, FF, and HP are available in lab packs, varying from 10 to 500 ml, for affinity purification of GST-tagged proteins using batch- or column-based methods. Glutathione Sepharos...
Keywords: Addition reactions, Buffers, Cell disruption, Centrifugation, Chromatography, Column chromatography, Gene expression, Homogenization, Oxidations, PAGE, Purification, Sample preparations, Sonication, Tagged proteins

Pull-down assays

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Cloning, Digestions, Gene expression, Genetic, Immobilization, Immunoprecipitation, Ligands, Mass spectrometry, PAGE, Precipitation

Troubleshooting Strategies in Purification of GST-tagged Proteins

This troubleshooting guide addresses the common problems associated with the majority of purification methods using the different Glutathione Sepharose media.
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Purification, Sonication, Tagged proteins, Western blot

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