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  • GE27-4577-01 - Anti-GST Antibody

GE27-4577-01 Sigma-Aldrich

Anti-GST Antibody

Cytiva, 27-4577-01

  •  NACRES NA.32



Related Categories Alphabetical Index, Antibodies, Antibodies to Fluorescent Labels, Biochemicals and Reagents, Fluorescent Probes, Labels, Particles and Stains,
conjugate   unconjugated
shipped in   wet ice
storage temp.   2-8°C
packaging   pkg of 0.5 mL, 50 detections
antibody form   affinity isolated antibody
antibody product type   primary antibodies
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mfr. no.   Cytiva, 27-4577-01


General description

Anti-GST Antibody is a polyclonal antibody purified from the sera of goats immunized with purified schistosomal GST. Because of its polyclonal nature, it can recognize more than one epitope on GST, thereby improving its capacity for recognizing GST fusion proteins even if some binding sites are masked due to recombinant protein folding.


Western Blots

Legal Information

GST Gene Fusion Vectors
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).

Sepharose is a registered trademark of Cytiva

Features and Benefits

• For the highly sensitive and specific detection of recombinant schistosomal GST fusion proteins expressed using pGEX vectors
• Extensively cross-adsorbed to remove antibodies that bind to E. coli proteins and affinity purified using Glutathione Sepharose®
• Supplied unconjugated for use with any enzyme-conjugated anti-goat antibody
• Recommended for use in Western blots and dot blots to detect GST fusion proteins

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

Refer to GE Healthcare Western Blotting Handbook (28-9998-97) for further information on optimization of antibody concentration for Western blotting.
Keywords: Tagged proteins, Western blot

Troubleshooting of Detection Methods of GST-tagged Proteins

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicate...
Keywords: Buffers, Cell disruption, Detection methods, Electroblotting, Gene expression, Purification, Tagged proteins, Western blot

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