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GE27-4590-01

GST Detection Module

Cytiva, 27-4590-01, sufficient for 50 detection reactions

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Properties

usage   sufficient for 50 detection reactions
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mfr. no.   Cytiva, 27-4590-01
packaging   pkg of 50 detections
shipped in   wet ice
storage temp.   2-8°C

Description

General description

GST detection module.

Application

In the biochemical assay, glutathione and 1-chloro-2-4-dinitrobenzene (CDNB) serve as substrates for GST to yield a yellow product detectable at 340 nm (1). An affinity-purified goat anti-GST polyclonal antibody suitable for Western blots is used in the immunoassay. The GST Detection Module contains sufficient components for 50 detection reactions using either assay: 1-chloro-2-4-dinitrobenzene, Glutathione, Anti-GST Antibody (see Anti-GST Antibody), and instruction booklet.

Features and Benefits

• GST Detection Module enables sensitive detection of GSTtagged Proteins and contains components
• For detection using either a biochemical assay where glutathione and 1-chloro-2-4-dititrobenzene (CDNB) serve as substrates for GST to yield a yellow product detectable at 340 nm, or an immunoassay.
• GST Detection Module contains components sufficient for 50 detection reactions using both assays, and an instruction booklet.

Analysis Note

www.gelifesciences.com.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Detection of GST-tagged Proteins

Several methods are available for detection of GST-tagged proteins, with method selection largely depending on the experimental situation. For example, SDS-PAGE analysis, although frequently used for...
Keywords: Affinity chromatography, Chromatography, Gene expression, PAGE, Tagged proteins, Western blot

GST Detection Module with CDNB Enzymatic Assay

GST-tagged proteins produced using pGEX vectors can be detected enzymatically using the GST substrate CDNB, included in the GST Detection Module. The GST-mediated reaction of CDNB with glutathione pr...
Keywords: Cell disruption, Tagged proteins

General considerations for purification of GST-tagged proteins

Yield of tagged protein is highly variable and is affected by the nature of the tagged protein, the host cell, and the expression and purification conditions used. Tagged protein yields can range from...
Keywords: Affinity chromatography, Cell disruption, Chromatography, Detection methods, Dialysis, Gene expression, Homogenization, PAGE, Sonication, Tagged proteins, Western blot

Solubilization of Membrane Proteins

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Crystallization, Degradations, Detergents, Dot blot, Enzyme activity, Filtration, Gene expression, Indicators, Ion Exchange, Ligands, PAGE, Purification, Separation, Solvents, Western blot

Troubleshooting Cleavage Methods

The troubleshooting guide below addresses problems common to the majority of cleavage methods as well as problems specific to a particular method. In the latter case, the relevant method is indicated....
Keywords: Affinity chromatography, Chromatography, Cloning, Electrophoresis, Tagged proteins, Western blot

Troubleshooting Detection Methods

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicated...
Keywords: Affinity chromatography, Buffers, Chromatography, Detection methods, Electroblotting, Gene expression, Tagged proteins, Western blot

Troubleshooting of Detection Methods of GST-tagged Proteins

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicate...
Keywords: Buffers, Cell disruption, Detection methods, Electroblotting, Gene expression, Purification, Tagged proteins, Western blot

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