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His GraviTrap Kit

Cytiva, 28-4013-51, pack of 10 columns

  •  NACRES NA.25



Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, GraviTrap Column Holder, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 10 columns
mfr. no.   Cytiva, 28-4013-51
bed volume   1 mL
capacity   40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
storage temp.   2-8°C


Analysis Note


Legal Information

GraviTrap is a trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Gravity-flow purification using His GraviTrap and His GraviTrap Kit

His GraviTrap™ columns are designed for fast and simple purification of histidine-tagged proteins using gravity flow. Both clarified and unclarified sample can be applied to the column. The column is pre...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, PAGE, Precipitation, Sequencing, Sonication, Tagged proteins, Western blot

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow

Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions. The ligand density of Ni Sep...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Homogenization, Immobilization, Ligands, Sample preparations, Sonication, Tagged proteins

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