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GE28-4017-45

GSTrap 4B

Cytiva, 28-4017-45, pack of 5 × 1 mL

  •  NACRES NA.56

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 × 1 mL
mfr. no.   Cytiva, 28-4017-45
parameter   <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi (H2O at 25°C)
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   7 mm
matrix   4% agarose
particle size   45-165 μm
average diameter   90 μm
cleaning   4 - 13
working range   4 - 13
capacity   >25 mg binding capacity (horse liver GST/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

Description

General description

GSTrap 4B columns are prepacked Glutathione Sepharose® 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

Application

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose® 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

Features and Benefits

• High binding capacity.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
• GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

www.gelifesciences.com.

Legal Information

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Glutathione Sepharose Products

Appendix 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Centrifugation, Chromatography, Inductively coupled plasma, Ligands, Purification, Tagged proteins

Characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (High Sub) Media and Columns

Glutathione Sepharose High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent. Glutathione Sepharose 4 Fast Flow is exce...
Keywords: Buffers, Centrifugation, Chromatography, Inductively coupled plasma, Ligands, Purification, Tagged proteins

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

The protocol below is an example optimized for 8 mg of target protein. It is worth estimating how much target protein is applied to the column, as this allows one to minimize the amount of protease a...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Digestions, Filtration, PAGE, Purification, Sonication, Tagged proteins

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

The protocol below is an example optimized for 8 mg of target protein. It is worth estimating how much target protein is applied to the column, as this allows one to minimize the amount of protease a...
Keywords: Absorption, Buffers, Chromatography, Digestions, Purification

High-throughput Screening Using GST MultiTrap FF and GST MultiTrap 4B 96-well Filter Plates

GST MultiTrap FF and GST MultiTrap 4B (Fig 3.4) are prepacked, disposable 96-well filter plates for reproducible, high-throughput screening of GST-tagged proteins. The plates are filled with a define...
Keywords: Buffers, Cell disruption, Centrifugation, Filtration, Gene expression, Oxidations, PAGE, Purification, Sample preparations, Sonication, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Purification Options for GST-tagged proteins using Glutathione Sepharose Products

Table 5.1 Purification options for GST-tagged proteins using Glutathione Sepharose Products
Keywords: Affinity chromatography, Chromatography, Purification, Tagged proteins

Purification Using GSTrap HP, GSTrap FF, and GSTrap 4B columns

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chrom...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Homogenization, Oxidations, PAGE, Purification, Sample preparations, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

Selecting Equipment for Purification of GST-tagged Proteins

The choice of equipment is dependent on the specific purification. Many purification procedures can be carried out using simple equipment and methods, for example, in a step-gradient elution method u...
Keywords: Chromatography, Purification

Selecting a GST Affinity Chromatography Product

A variety of affinity chromatography products are available from GE Healthcare that have glutathione immobilized as ligand: Sepharose High Performance (HP), Sepharose 4 Fast Flow (FF), and Sepharose ...
Keywords: Affinity chromatography, Cell disruption, Chromatography, Immobilization, Ligands, Purification, Tagged proteins

Troubleshooting Cleavage Methods

The troubleshooting guide below addresses problems common to the majority of cleavage methods as well as problems specific to a particular method. In the latter case, the relevant method is indicated....
Keywords: Affinity chromatography, Chromatography, Cloning, Electrophoresis, Tagged proteins, Western blot

Troubleshooting Purification Methods

The troubleshooting guide below addresses problems common to the majority of purification methods as well as problems specific to a particular method. In the latter case, the relevant method is indicat...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Sonication, Tagged proteins, Western blot

Troubleshooting Strategies in Purification of GST-tagged Proteins

This troubleshooting guide addresses the common problems associated with the majority of purification methods using the different Glutathione Sepharose media.
Keywords: Buffers, Cell disruption, Centrifugation, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Purification, Sonication, Tagged proteins, Western blot

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