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GE28-9034-70

GFX PCR DNA and Gel Band Purification Kit

Cytiva, 28-9034-70

  •  NACRES NA.52

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Properties

Related Categories DNA & RNA Purification, Molecular Biology, Post-Reaction Clean-up More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 100 ea
mfr. no.   Cytiva, 28-9034-70

Description

General description

Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μ or agarose gel slices up to 900 mg.

illustra GFX PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μ or agarose gel slices up to 900 mg. The kit combines a versatile chaotropic buffer with a glass fiber matrix supported in a spin column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 60% to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed.

illustra GFX PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX columns, collection tubes, color-coded bottles of capture buffer, wash buffer, and two elution buffers (Tris-HCl and sterile water), and an instruction booklet.

Features and Benefits

• For the isolation and concentration of DNA fragments (50 bp to 40 kb) from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.
• Fast and easy-to-use method with less than 10 min hands-on time.
• Flexible 10 to 50 μ elution volume for different DNA concentration needs.
• Improved column design with increased maximum volume, holding of up to 800 μ liquid volume (950 μ without a cap).
• Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.
• Addition of a visual color indicator to the capture buffer ensures optimal pH for maximum DNA binding and recovery.
• Two choices of elution buffer provided to fit downstream applications.
• Color-coded caps for ease of use.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GFX is a trademark of Cytiva

illustra is a trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

DNA Cleanup using illustra™ GFX Kits

Cytiva provides three silica-based kits for DNA cleanup. illustra™ GFX™ PCR and Gel Band Purification Kit purifies PCR products and other DNAs from solution or gel slices. This product is discussed i...
Keywords: Adsorption, Amplification, Buffers, Centrifugation, Cloning, Digestions, Microarray Analysis, Peptide synthesis, Polymerase chain reaction, Purification, Sequencing

Downstream Applications for Plasmid DNA and Substrates

The purity of plasmid DNA in terms of protein, salt, particulates, RNA, and endotoxin contamination, and quality measures such as the percentage of supercoiled plasmid DNA, will all affect downstream...
Keywords: AGE, Amplification, Cloning, Digestions, Electrophoresis, Enzyme activity, Gel electrophoresis, Indicators, Molecular biology, Peptide synthesis, Polymerase chain reaction, Purification, Recombination, Sequencing, Solvents, Transfection

Possible Causes and Solutions for Problems that may occur during Plasmid DNA Preparation

Table 5.6. Possible causes and solutions for problems that may occur during plasmid DNA preparation1.
Keywords: Buffers, Centrifugation, DNA purification, Purification

Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit

A general protocol for purification and concentration of DNA from PCR mixtures, restriction enzyme digests, and solutions using illustra™ GFX™ 96 PCR Purification Kit is provided below. The kit can b...
Keywords: Buffers, Centrifugation, Filtration, Peptide synthesis, Polymerase chain reaction, Purification

Restriction Digestion of pGEX Vectors

Many restriction enzymes are compatible with OPA+, and its recipe is provided here as a convenience. The buffer is also recommended for use in the dephosphorylation and ligation procedures that follo...
Keywords: AGE, Digestions, Electrophoresis, Gel electrophoresis, Peptide synthesis, Polymerase chain reaction, Purification

Peer-Reviewed Papers
15

References

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