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GE28-9187-80

MBPTrap HP

Cytiva, 28-9187-80, pack of 5 × 5 mL

  •  NACRES NA.56

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Properties

Related Categories Affinity Chromatography, Carbohydrate Matrices, Molecular Biology, Protein Chromatography, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 5 × 5 mL
mfr. no.   Cytiva, 28-9187-80
parameter   <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
  42 psi
bed size   16 mm × 25 mm
bed volume   5 mL
column I.D.   16 mm
matrix   highly cross-linked 6% agarose
avg. part. size   34 μm
cleaning   2 - 13
working range   >7
capacity   ~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
storage temp.   2-8°C

Description

General description

MBPTrap HP columns prepacked with Dextrin Sepharose® High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP).

Features and Benefits

• HiTrap® columns prepacked with Dextrin Sepharose® High Performance medium.
• Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
• Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
• Physiological conditions and mild elution preserve the activity of the target protein.
• Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

HiTrap is a registered trademark of Cytiva

MBPTrap is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Dextrin Sepharose High Performance Products

Appendix 3, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Chromatography, Ligands, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Purification using MBPTrap HP Columns

MBPTrap HP 1 ml and 5 ml columns (Fig 6.2) are made of biocompatible polypropylene that does not interact with biomolecules. Prepacked MBPTrap HP columns provide fast, simple, and easy separations in...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Homogenization, PAGE, Sample preparations, Size-exclusion chromatography, Sonication, Tagged proteins

Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins

From our library of Protocols, Sigma-Aldrich presents Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Detergents, Gene expression, Phase transitions, Precipitation, Sonication, Tagged proteins

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