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GE28-9523-61

GST Buffer Kit

Cytiva, 28-9523-61, pack of 1 kit

  •  NACRES NA.56

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Properties

shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 kit
mfr. no.   Cytiva, 28-9523-61
shipped in   wet ice
storage temp.   2-8°C

Description

Analysis Note

www.gelifesciences.com.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (High Sub) Media and Columns

Glutathione Sepharose High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent. Glutathione Sepharose 4 Fast Flow is exce...
Keywords: Buffers, Centrifugation, Chromatography, Inductively coupled plasma, Ligands, Purification, Tagged proteins

Gravity-Flow Purification Using GST GraviTrap

GST GraviTrap™ is designed for fast and simple purification of GST-tagged proteins using gravity flow. The column is prepacked with 2 ml of Glutathione Sepharose® 4B, enough to purify up to 50 mg of rG...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Chromatography, Homogenization, Oxidations, Sample preparations, Sonication, Tagged proteins

Gravity-flow Purification Using GST GraviTrap

GST GraviTrap is designed for fast and simple purification of GST-tagged proteins using gravity flow. The column is prepacked with 2 ml of Glutathione Sepharose 4B, enough to purify up to 50 mg of GS...
Keywords: Buffers, Cell culture, Homogenization, Oxidations, PAGE, Purification, Sample preparations, Sonication, Tagged proteins

Minipreps Using GST SpinTrap

GST SpinTrap™ columns are designed for rapid, small-scale purification of GST-tagged proteins using conditions of mild affinity purification. Greater than 90% purity can be achieved in a single step. Th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Gene expression, Homogenization, Oxidations, Protein extraction, Sample preparations, Sonication, Tagged proteins

Pull-down assays

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Cloning, Digestions, Gene expression, Genetic, Immobilization, Immunoprecipitation, Ligands, Mass spectrometry, PAGE, Precipitation

Purification Options for GST-tagged proteins using Glutathione Sepharose Products

Table 5.1 Purification options for GST-tagged proteins using Glutathione Sepharose Products
Keywords: Affinity chromatography, Chromatography, Purification, Tagged proteins

Purification Using GST SpinTrap

GST SpinTrap columns are designed for rapid, small-scale purification of GST-tagged proteins using mild conditions of affinity purification. More than 90% purity can be achieved in a single step. The...
Keywords: Addition reactions, Buffers, Cell culture, Cell disruption, Centrifugation, Gene expression, Homogenization, Oxidations, PAGE, Protein extraction, Purification, Sample preparations, Sonication, Tagged proteins

Selecting Equipment for Purification of GST-tagged Proteins

The choice of equipment is dependent on the specific purification. Many purification procedures can be carried out using simple equipment and methods, for example, in a step-gradient elution method u...
Keywords: Chromatography, Purification

Troubleshooting Purification Methods

The troubleshooting guide below addresses problems common to the majority of purification methods as well as problems specific to a particular method. In the latter case, the relevant method is indicat...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Degradations, Detergents, Electrophoresis, Gene expression, Ion Exchange, Precipitation, Sonication, Tagged proteins, Western blot

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