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TALON® Superflow

Cytiva, 28-9574-99, pack of 10 mL

  •  NACRES NA.56



shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 10 mL
mfr. no.   Cytiva, 28-9574-99
matrix   6% cross-linked agarose
particle size   60-160 μm
cleaning in place   2 - 14 (Co2+-stripped medium)
working range   3 - 12 (Co2+-stripped medium)
capacity   ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.   2-8°C


General description

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media.

Features and Benefits

• Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
• Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
• Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
• Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Superflow is a trademark of Sterogene Bioseparations Inc.

Talon is a registered trademark of Clontech

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Protocols & Articles


Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Ni Sepharose® High Performance is recommended for high-resolution purification of histidine-tagged proteins, providing sharp peaks and concentrated eluate. Ni Sepharose® 6 Fast Flow is excellent for s...
Keywords: Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography

Manual Purification of Histidine-Tagged Proteins using His GraviTrap TALON

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins. The different purification ...
Keywords: Buffers, Cell disruption, PAGE, Tagged proteins

Optimizing Purification of Histidine-Tagged Proteins

The presence of surface-exposed histidine residues or other complex-forming amino acids can lead to unwanted binding of untagged host cell proteins to purification media. These untagged proteins may e...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Immobilization, Ion Exchange, PAGE, Purification, Separation, Sequencing, Size-exclusion chromatography, Tagged proteins

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and ...
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Filtration, Homogenization, Precipitation, Sample preparations, Sonication, Tagged proteins

Purification of Histidine-Tagged Proteins using HiTrap® TALON® Crude

HiTrap® TALON® crude is 1 ml and 5 ml HiTrap® columns prepacked with TALON® Superflow. The columns can be operated with either a syringe, a peristaltic pump, or a chromatography system such as ÄKTA. A...
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, PAGE

Purification of Histidine-Tagged Proteins using His MultiTrap TALON

His MultiTrap™ TALON® 96-well filter plates are prepacked with 50 µl of TALON® Superflow per well. Each plate provides highly reproducible high-throughput screening and rapid small-scale purification of...
Keywords: Cell disruption, Centrifugation, Gene expression, Tagged proteins

Purification of Histidine-Tagged Proteins using TALON Superflow

TALON® Superflow consists of highly cross-linked agarose beads with an immobilized chelating group precharged with Co2+ ions.
Keywords: Buffers, Cell disruption, Chromatography, Filtration, Gene expression, Homogenization, Immobilization, Sample preparations, Sonication, Tagged proteins

Rapid Screening of Histidine-Tagged Proteins using His SpinTrap™ TALON®

His SpinTrap™ TALON® columns are excellent for screening of expression levels and purification conditions prior to scale up. The columns are designed for use in a microcentrifuge. Figure 3.40 shows th...
Keywords: Cell disruption, Centrifugation, Gene expression, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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