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GE28-9852-55

Protein G GraviTrap

Cytiva, 28-9852-55, pack of 10 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, GE Healthcare Life Sciences Protein Sample Preparation Formats, GraviTrap Column Holder, Molecular Biology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 10 × 1 mL
mfr. no.   Cytiva, 28-9852-55
bed volume   1 mL
capacity   ~20 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
storage temp.   2-8°C

Description

General description

Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 mL of Protein G Sepharose® 4 Fast Flow.

Protein G GraviTrap are prepacked gravity-flow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on protein G ligands in gravity-flow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Application

Fast and efficient manual purification of monoclonal and polyclonal antibodies

Features and Benefits

• Prepacked gravity-flow columns provide convenience, save time, and increase the reliability of your results.
• Purification of antibodies in 30 min allows you to quickly obtain and store your target protein in order to avoid the inherent dangers of protein instability and degradation associated with slow and long purification runs.
• High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
• High binding capacity such as Protein G GraviTrap ~ 20 mg human IgG/column

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

GraviTrap is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

Protein G GraviTrap™ are gravity-flow columns prepacked with 1 ml of Protein G Sepharose® 4 Fast Flow. The columns are designed for fast and efficient manual purification of monoclonal and polyclonal an...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, Sample preparations

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