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GE28-9852-56

rProtein A/Protein G GraviTrap

Cytiva, 28-9852-56, pack of 10 × 1 mL

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, GraviTrap Column Holder, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 10 × 1 mL
mfr. no.   Cytiva, 28-9852-56
bed volume   1 mL
capacity   35 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)
storage temp.   2-8°C

Description

General description

rProtein A Protein G GraviTrap includes ten ready-to-use columns prepacked with 1 mL of a mixture of 50% rProtein A Sepharose® Fast FLow and 50% protein G Sepharose® 4 Fast Flow.

Application

rProtein A Protein G GraviTrap are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A and Protein G ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Features and Benefits

• Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
• Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
• High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
• High binding capacity such as Protein A/Protein G GraviTrap ~ 35 mg human IgG/column.

Analysis Note

www.gelifesciences.com.

Legal Information

GraviTrap is a trademark of Cytiva

Sepharose is a registered trademark of Cytiva

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

Protein G GraviTrap™ are gravity-flow columns prepacked with 1 ml of Protein G Sepharose® 4 Fast Flow. The columns are designed for fast and efficient manual purification of monoclonal and polyclonal an...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Ligands, Sample preparations

Performing a Purification of IgG Antibodies with rProtein A GraviTrap and rProtein A/Protein G GraviTrap

rProtein A GraviTrap™ are gravity-flow columns prepacked with 1 ml of rProtein A Sepharose® 4 Fast Flow. The columns are designed for fast and efficient manual purification of monoclonal and polyclonal ...
Keywords: Affinity chromatography, Cell culture, Chromatography, Ligands, Sample preparations

Purification using protein A chromatography media

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose® so that these region...
Keywords: Affinity chromatography, Buffers, Chromatography, Fermentation, Immunoprecipitation, Ligands, Purification, Size-exclusion chromatography

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