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GE28-9889-38 Sigma-Aldrich

XK 16/40 Column

  •  NACRES SB.52

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Description

General description

XK empty columns are designed for liquid chromatography at low to medium pressure. The user-friendly design ensures trouble-free operation with fittings for direct connection to ÄKTA design instruments or other high-performance liquid chromatography systems. The product family is based on a robust design for high reproducibility and accurate results. XK columns are compatible with aqueous solutions and most organic solvents used in liquid chromatography of macromolecules.

XK empty columns provide reliable and reproducible separations. The tube material is of borosilicate glass, which allows for visual inspection of the media bed. The columns are made of biocompatible materials that exhibit excellent chemical resistance. A broad range of column dimensions makes XK columns suitable for many different purification needs. Column packing can be performed using either a packing reservoir or an extra column tube attached with a packing connector. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments of the bed height, disassembly, and cleaning. The design of the adapter plunger gives a uniform flow, which maintains the integrity of the packed bed during operations. An extensive range of spare parts is available, facilitating maintenance.

Features and Benefits

Adapter QuickLock mechanism that facilitates column handling and cleaning

Cooling jacket for sensitive purifications

Fittings for direct connection to chromatographiy systems

Plunger design to ensure a homogeneous buffer distribution and reproducible packing

User friendly and robust empty columns, designed for liquid chromatography at low to medium pressure.

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Safety & Documentation

Safety Information

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Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Column Packing and Preparation for Affinity Chromatography of Antibodies

Use small prepacked columns for chromatography media scouting and method optimization and to increase efficiency in method development.
Keywords: Antimicrobials, Chromatography, Detergents, Gene expression, Separation, Solvents

Converting from Flow Velocity to Volumetric Flow Rates

It is convenient when comparing results for columns of different sizes to express flow as flow velocity (cm/h). However, flow is usually measured in volumetric flow rate (ml/min). To convert between flow ...

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer ...
Keywords: Absorption, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Electrophoresis, Fractionation, Gel electrophoresis, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Titrations

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

Protein G Sepharose® 4 Fast Flow consists of 90 µm beads of highly cross-linked agarose, which provide a robust and stable chromatography matrix that allows high flow rates. The medium is a good choi...
Keywords: Buffers, Chromatography, Purification, Sample preparations, Separation

Performing a Purification of IgG Antibodies with rProtein A Sepharose Fast Flow

rProtein A Sepharose® Fast Flow (Figure 3.19) is an affinity medium with high binding capacity for monoclonal and polyclonal antibodies. The binding capacity of rProtein A Sepharose® Fast Flow is cons...
Keywords: Buffers, Fermentation, Ligands, Sample preparations

Protocols

Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

This appendix provides column packing guidelines for affinity chromatography media. Efficient column packing is essential for a good separation, especially when using gradient elution.
Keywords: Affinity chromatography, Buffers, Chromatography, Purification, Separation

Column Packing and Preparation

Prepacked columns from Cytiva will ensure reproducible results and the highest performance.
Keywords: Antimicrobials, Chromatography, Gene expression, Separation

Column Packing and Preparation

A well-packed column is essential for a high-resolution fractionation on any SEC medium. Prepacked columns from Cytiva will ensure reproducible results and the highest performance. If the column volu...
Keywords: Buffers, Chromatography, Filtration, Fractionation, Separation, Size-exclusion chromatography

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose Excel

Ni Sepharose excel consists of 90 µm highly cross-linked agarose beads, to which a chelating ligand has been coupled. The ligand is precharged with nickel ions that are exceptionally strongly bound, ...
Keywords: Buffers, Cell culture, Centrifugation, Chromatography, Dialysis, Ligands, Precipitation, Sample preparations, Tagged proteins

Purification of Histidine-Tagged Proteins using TALON Superflow

TALON® Superflow consists of highly cross-linked agarose beads with an immobilized chelating group precharged with Co2+ ions.
Keywords: Buffers, Cell disruption, Chromatography, Filtration, Gene expression, Homogenization, Immobilization, Sample preparations, Sonication, Tagged proteins

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