• USA Home
  • GE28-9893-36 - HiLoad® 26/600 Superdex® 200 pg

EMAIL THIS PAGE TO A FRIEND
GE28-9893-36 Sigma-Aldrich

HiLoad® 26/600 Superdex® 200 pg

GE Healthcare, 28-9893-36, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

  •  NACRES SB.52

Purchase

Properties

Related Categories Analytical/Chromatography, GE Life Sciences Gel Filtration Columns, Gel Filtration Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC,
material   bed support (polyamide)
  borosilicate glass column
  glass fiber (polypropylene reinforced)
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
mol wt   10000-600000 Da (globular proteins)
packaging   pkg of 1 ea
mfr. no.   GE Healthcare, 28-9893-36
parameter   <2.6 mL/min flow rate
  0.3 Mpa
bed volume   320 mL
column L × I.D.   60 cm × 26 mm
sample volume   <13 mL
matrix   agarose-dextran composite
  dextran-agarose particle platform
matrix active group   dextran-agarose phase
  dextran-agarose phase
avg. part. size   34 μm
cleaning   1 - 14
working range   3 - 12
separation technique   size exclusion (SEC)

Description

General description

HiLoad® 26/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice highly suitable for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Features and Benefits

• High resolution with short run times and good recovery.
• High capacity at high flow rates.
• Separations can be scaled up to production levels.
• Prepacked for convenience and reproducibility.
• 1/16” fittings for convenient connections to AKTA design systems.

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiLoad is a registered trademark of GE Healthcare

Superdex is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Protocols

Performing a Separation with Superdex

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Buffers, Chromatography, Detergents, Separation

Sample Preparation for Size Exclusion Chromatography

Appendix 3, Extracted from Size Exclusion Chromatography Principles and Methods (PDF) GE Healthcare, 2014
Keywords: Buffers, Centrifugation, Chromatography, Detergents, Electrophoresis, Filtration, Nucleic acid denaturation, Precipitation, Purification, Sample preparations, Tagged proteins

Separation Options with Superdex

Extracted from Size Exclusion Chromatography Principles and Methods, (PDF) GE Healthcare, 2014
Keywords: Chromatography, Fractionation, Purification, Separation

Setting Column Pressure Limits for Size Exclusion Chromatography

Extracted from Size Exclusion Chromatography Principles and Methods (PDF) GE Healthcare, 2014
Keywords: Chromatography

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?