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GE29-0005-94

His GraviTrap TALON®

Cytiva, 29-0005-94, pack of 10 columns

  •  NACRES NA.56

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Properties

Related Categories GE Healthcare Life Sciences Protein Sample Preparation Formats, GraviTrap Column Holder, Molecular Biology, Native Protein Sample Preparation, Proteomics More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 10 columns
mfr. no.   Cytiva, 29-0005-94
bed volume   1 mL
column I.D.   15 mm
matrix   6% cross-linked agarose
particle size   60-160 μm
cleaning   2 - 14 (Co2+-stripped medium)
working range   3 - 12 (Co2+-stripped medium)
capacity   ≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
  ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.   2-8°C

Description

General description

His GraviTrap Talon® is a prepacked gravity f low column for convenient manual purification of histidine-tagged proteins.

Application

His GraviTrap Talon® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged Proteins directly from clarified or unclarified cell lysates. His GraviTrap Talon® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.

Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Features and Benefits

• Convenient histidine-tagged purification with high purity (> 90%) and binding capacity of up to 15 mg/column.
• Ready-to-use gravity flow column prepacked with 1 mL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
• One run takes about 30 minutes, and large sample volumes can be applied all at once.

Analysis Note

www.gelifesciences.com.

Legal Information

GraviTrap is a trademark of Cytiva

Talon is a registered trademark of Clontech

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Manual Purification of Histidine-Tagged Proteins using His GraviTrap TALON

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins. The different purification ...
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, PAGE, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Purification of Histidine-Tagged Proteins using TALON Superflow

TALON® Superflow consists of highly cross-linked agarose beads with an immobilized chelating group precharged with Co2+ ions.
Keywords: Affinity chromatography, Buffers, Cell disruption, Chromatography, Filtration, Gene expression, Homogenization, Immobilization, Sample preparations, Sonication, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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