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GE29-0485-86

HisTrap Excel

Cytiva, 29-0485-86, pack of 1 mL

  •  NACRES NA.56

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Properties

Related Categories Additional Affinity Purification, Affinity Chromatography, IMAC Matrices, Molecular Biology, Plant Biotechnology,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 mL
mfr. no.   Cytiva, 29-0485-86
availability   not available in North America
parameter   1 ml/min - 4 ml/min flow rate (H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.)
  5 bar
bed size   7 mm × 25 mm
bed volume   1 mL
column I.D.   7 mm
matrix   highly cross-linked 6% agarose
average diameter   90 μm
cleaning   2 - 14 (Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
working range   2 - 13
capacity   ≥10 mg binding capacity (histidine-tagged protein)(H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.)
  ≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)

Description

General description

HisTrap excel columns are prepacked with Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted by immobilized metal ion affinity chromatography (IMAC). The media is resistant to chelating and reducing agents making the medium an excellent choice for the purification of excreted proteins.

Features and Benefits

• HiTrap columns prepacked with Ni Sepharose excel medium, resistant to chelating and reducing agents
• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
• Increase target protein yield and decrease degradation through reduced and simplified sample handling
• HisTrap excel columns allow direct purification of cell-free, unclarified samples.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

HisTrap is a trademark of Cytiva

Safety & Documentation

Safety Information

Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NA 1993 / PGIII

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and Uncharged IMAC Sepharose Products

Appendix 1, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016  
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Detergents, Immobilization, Precipitation, Purification, Tagged proteins

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

Desalting at laboratory scale is a well-proven, simple, and very fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired bu...
Keywords: Absorption, Addition reactions, Affinity chromatography, Buffers, Centrifugation, Chromatography, Detergents, Dialysis, Fractionation, Ion Exchange, PAGE, Precipitation, Sample preparations, Separation, Size-exclusion chromatography, Tagged proteins

Handling Inclusion Bodies

Recombinant proteins are most often expressed in the intracellular space, but expression can also be controlled so that the protein is secreted into the periplasmic space or out into the culture medi...
Keywords: Affinity chromatography, Buffers, Chromatography, Detergents, Dialysis, Enzyme activity, Fractionation, Gene expression, Immobilization, Mass spectrometry, Nuclear magnetic resonance spectroscopy, PAGE, Purification, Reversed-phase chromatography, Sample preparations, Separation, Size-exclusion chromatography, Solvents, Sonication, Tagged proteins

Manual and Automated Purification

Chapter 2, Extracted from Affinity Chromatography Vol. 2: Tagged Proteins, GE Healthcare, 2016
Keywords: Affinity chromatography, Centrifugation, Chromatography, Gene expression, Tagged proteins

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and ...
Keywords: Affinity chromatography, Buffers, Cell disruption, Centrifugation, Chromatography, Filtration, Homogenization, Precipitation, Sample preparations, Sonication, Tagged proteins

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel

HisTrap excel 1 ml and 5 ml columns are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the medium enables fast an...
Keywords: Affinity chromatography, Buffers, Cell culture, Chromatography, Degradations, Gene expression, PAGE, Tagged proteins

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose Excel

Ni Sepharose excel consists of 90 µm highly cross-linked agarose beads, to which a chelating ligand has been coupled. The ligand is precharged with nickel ions that are exceptionally strongly bound, ...
Keywords: Affinity chromatography, Buffers, Cell culture, Centrifugation, Chromatography, Dialysis, Ligands, Precipitation, Sample preparations, Tagged proteins

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

The troubleshooting guide below addresses problems common to the majority of purification products discussed in this chapter, as well as problems specific to a particular method. In the latter case, th...
Keywords: Addition reactions, Affinity chromatography, Buffers, Cell culture, Cell disruption, Centrifugation, Chromatography, Detergents, Fermentation, PAGE, Precipitation, Protein biosynthesis, Size-exclusion chromatography, Sonication, Tagged proteins, Western blot

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