• USA Home
  • GERPN2105 - ECL Detection Reagents

GERPN2105 Sigma-Aldrich

ECL Detection Reagents

  •  NACRES NA.32



Related Categories Biochemicals and Reagents, Chemiluminescent compounds, Core Bioreagents, General Reagents, Life Science Reagents for Western Blotting,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 kit
mfr. no.   GE Healthcare, RPN2105
storage temp.   2-8°C


General description

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

ECL is a trademark of GE Healthcare

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?