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GERPN2108 Sigma

ECL Western Blotting Analysis System

GE Healthcare, RPN2108

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Properties

Related Categories Biochemicals and Reagents, Chemiluminescent compounds, Core Bioreagents, Detection Substrates, ECL Detection Reagents,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   wetted part: no
packaging   pkg of 1 kit
mfr. no.   GE Healthcare, RPN2108
color   Detection reagent 1: Colorless Detection reagent 2: Colorless Peroxidase labelled anti-mouse antibody: Colorless Peroxidase labelled anti-rabbit antibody: Colorless Blocking agent: White
storage temp.   2-8°C

Description

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Application

ECL Blotting Analysis System has been used in western blotting.

Western Blotting

Features and Benefits

• Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.
• Optimized system provides rapid, sensitive results using simple protocols.
• Designed for use with nitrocellulose and PVDF membranes.
• High sensitivity: Up to 10 times more sensitive than colorimetric methods.
• Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
• Easy reprobing: Save time and materials by reprobing on membranes up to 10 times.
• Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
• Non-radioactive-no specialized facilities needed
• Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide

General description

ECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system.

ECL Western Blotting System uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.

Each system contains the following reagents, sufficient for detection of 1000 cm2 membrane:
Anti-mouse HRP conjugate, 100 μl
Anti-rabbit HRP conjugate, 100 μl
ECL Detection Reagents 1 and 2, 62.5 mL each
Blocking agent, 5 g.

Legal Information

ECL is a trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

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