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ECL Blotting Reagents

Cytiva, RPN2109

  •  NACRES NA.32



Related Categories Biochemicals and Reagents, Chemiluminescent compounds, Core Bioreagents, Detection Substrates, ECL Detection Reagents,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   wetted part: no
packaging   pkg of 1 kit
mfr. no.   Cytiva, RPN2109
color   Detection reagent 1: Colorless Detection reagent 2: Colorless
storage temp.   2-8°C


General description

Amersham ECL Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL Western Blotting Detection Reagents in combination with Hybond® ECL membranes form a complete solution for Western blot applications.

Contents include the following:
GERPN2109, 62.5 mL each of ECL Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2).


Western Blotting

Features and Benefits

• High sensitivity, ~10 pg in model system.
• Proven performance, the most frequently cited ECL Western Blotting Detection Reagents in publications
• Detectable on Hyperfilm ECL or with CCD imager
• Recommended membrane Hybond ECL


Sufficient for detection of 1000 cm2 of membrane:
62.5 mL Detection reagent 1(color : colorless)
62.5 mL Detection reagent 2 (color: colorless)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Amersham is a trademark of Cytiva

ECL is a trademark of Cytiva

Hybond is a registered trademark of Cytiva

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


GE Western Blotting Introduction

Introduction, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011
Keywords: Catalytic combustion detector, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Immobilization, Oxidations, Purification, Sample preparations, Separation, Western blot


Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

Refer to GE Healthcare Western Blotting Handbook (28-9998-97) for further information on optimization of antibody concentration for Western blotting.
Keywords: Tagged proteins, Western blot

Detection of GST-tagged Proteins with Western Blot

Expression and purification of GST-tagged proteins can be monitored by Western blot analysis, using Amersham ECL, Amersham ECL Prime, or Amersham ECL Select detection systems to enhance sensitivity.
Keywords: Electrophoresis, Gene expression, PAGE, Purification, Separation, Tagged proteins, Western blot

Troubleshooting of Detection Methods of GST-tagged Proteins

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicate...
Keywords: Buffers, Cell disruption, Detection methods, Electroblotting, Gene expression, Purification, Tagged proteins, Western blot

Peer-Reviewed Papers


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