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GERPN2232 Sigma-Aldrich

ECL Prime Western Blotting System

GE Healthcare, RPN2232

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Chemiluminescent compounds, Core Bioreagents, Detection Substrates, ECL Detection Reagents,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 kit
mfr. no.   GE Healthcare, RPN2232
color   Detection reagent 1: Colorless Detection reagent 2: Colorless

Description

General description

Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.

Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

Application

Sensitive quantitative or confirmatory Western Blotting

Features and Benefits

• High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
• Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
• Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm.
• Compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers.
• Optimized for use with Amersham Hybond-P (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
• Recommended membrane Hybond-P or Hybon ECL

Components

The following components are included in the Amersham ECL Prime detection reagent kit.
• RPN2232 Solution A: Luminol solution, 50 ml Solution B: Peroxide solution, 50 ml sufficient for 1000 cm2 membrane

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

ECL Prime
This product or portions thereof is manufactured and sold under license from Cyanagen Srl and is subject of US patent 7,855,287, US Patent 7,803,573, and Italian application number TO2010A000580, together with other equivalent granted patents and patent applications in other countries.

Amersham is a trademark of GE Healthcare

ECL is a trademark of GE Healthcare

Rainbow is a trademark of GE Healthcare

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Articles

GE Western Blotting Introduction

Introduction, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011
Keywords: Catalytic combustion detector, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Immobilization, Oxidations, Purification, Sample preparations, Separation, Western blot

Protocols

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

Refer to GE Healthcare Western Blotting Handbook (28-9998-97) for further information on optimization of antibody concentration for Western blotting.
Keywords: Tagged proteins, Western blot

Detection of GST-tagged Proteins with Western Blot

Expression and purification of GST-tagged proteins can be monitored by Western blot analysis, using Amersham ECL, Amersham ECL Prime, or Amersham ECL Select detection systems to enhance sensitivity.
Keywords: Electrophoresis, Gene expression, PAGE, Purification, Separation, Tagged proteins, Western blot

Troubleshooting of Detection Methods of GST-tagged Proteins

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicate...
Keywords: Buffers, Cell disruption, Detection methods, Electroblotting, Gene expression, Purification, Tagged proteins, Western blot

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