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GERPN2235 Sigma-Aldrich

ECL Select Western Blotting Detection Reagent

GE Healthcare, RPN2235

  •  NACRES NA.32



Related Categories Biochemicals and Reagents, Chemiluminescent compounds, Core Bioreagents, Detection Substrates, ECL Detection Reagents,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 kit
mfr. no.   GE Healthcare, RPN2235


General description

Amersham ECL Select: Western blotting detection reagent with highly intense signal output, for detection of medium to very low protein levels.

Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham ECL Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.

Amersham ECL Select typically exhibits a dynamic range that is complementary to Amersham ECL Prime, and similar to Amersham ECL Advance.


Western Blotting

Features and Benefits

• Improved sensitivity: Produces a very high signal intensity
• Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins
• Works with a wide range of antibody dilutions, reducing the need for lengthy optimization
• Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham Hyperfilm
• Compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers.
• Optimized for use with Amersham Hybond-P (PVDF) membranes and compatible with Amersham Hybond® ECL (nitrocellulose) membranes


Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane

complete packsize
for 1000 cm2 membrane

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

Amersham is a trademark of GE Healthcare

ECL is a trademark of GE Healthcare

Hybond is a registered trademark of GE Healthcare Companies

Rainbow is a trademark of GE Healthcare

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


GE Western Blotting Introduction

Introduction, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011
Keywords: Catalytic combustion detector, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Immobilization, Oxidations, Purification, Sample preparations, Separation, Western blot

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot


Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

Refer to GE Healthcare Western Blotting Handbook (28-9998-97) for further information on optimization of antibody concentration for Western blotting.
Keywords: Tagged proteins, Western blot

Detection of GST-tagged Proteins with Western Blot

Expression and purification of GST-tagged proteins can be monitored by Western blot analysis, using Amersham ECL, Amersham ECL Prime, or Amersham ECL Select detection systems to enhance sensitivity.
Keywords: Electrophoresis, Gene expression, PAGE, Purification, Separation, Tagged proteins, Western blot

Troubleshooting of Detection Methods of GST-tagged Proteins

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicate...
Keywords: Buffers, Cell disruption, Detection methods, Electroblotting, Gene expression, Purification, Tagged proteins, Western blot

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