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GERPN3005 Sigma-Aldrich

ECL Direct Nucleic Acid

GE Healthcare, RPN3005, pack of 1 ea

  •  NACRES NA.31

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Properties

Related Categories Molecular Biology, Nucleic Acid Detection and Hybridization, Nucleic Acid Enzymes and Reagents More...
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pack of 1 ea
mfr. no.   GE Healthcare, RPN3005
storage temp.   2-8°C

Description

General description

ECL Direct Labeling Module.

Application

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL Detection Reagents, and ECL Gold Hybridization Buffer.

Analysis Note

www.gelifesciences.com.

Legal Information

ECL is a trademark of GE Healthcare

Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

Peer-Reviewed Papers
15

References

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