• USA Home
  • GERPN5785 - Vistra ECF Substrate


Vistra ECF Substrate

Cytiva, RPN5785

  •  NACRES NA.32



Related Categories Biochemicals and Reagents, Buffers and Reagents, Buffers, Reagents, & Stains, Chemiluminescent compounds, Core Bioreagents,
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging   pkg of 1 kit
mfr. no.   Cytiva, RPN5785
color   ECF substrate: Off-white/white to yellowish ECF substrate dilution buffer: Colorless
shipped in   wet ice
storage temp.   2-8°C


General description

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF Substrate, 60 mL, and dilution buffer.


Vistra ECF substrate has been used in Western blotting.

Features and Benefits

• A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
• For use in western blotting, dot, and slot blotting applications
• Sensitivity comparable to chemiluminescence
• The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
• Eliminates need for darkroom and film

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Legal Information

ECF is a trademark of Cytiva

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


GE Western Blotting Introduction

Introduction, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011
Keywords: Catalytic combustion detector, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Immobilization, Oxidations, Purification, Sample preparations, Separation, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?