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GERPN800E Sigma

Amersham ECL Rainbow Marker - Full range

Full Range, GE Healthcare, RPN800E

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Properties

Related Categories Core Bioreagents, Life Science Reagents for Western Blotting, Markers, Molecular Biology, Molecular Weight Markers,
form   liquid
shelf life   Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature   wetted part: no
  Full Range
packaging   pkg of 250 μL
mfr. no.   GE Healthcare, RPN800E
color   Brownish-red
solubility   cold water: soluble (Easily soluble in cold and hot water)
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Application

For industrial applications: Analytical reagent, research
Amersham ECL Rainbow Marker - Full range has been used as a molecular weight marker for immunoblotting and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Features and Benefits

• Monitor progress of protein electrophoresis and assess transfer efficiency and molecular weight of blotted proteins.without staining
• High intensity, prestained multicolored bands allow distinction of closely related proteins.
• Full-Range Rainbow Markers (Mr 12 000 to 225 000) use ten separate proteins with six different colors.
• High-Range Rainbow Markers (Mr 12 000 to 225 000) use eight separate proteins with six different colors.
• Low-Range Rainbow Markers (Mr 3 500 to 38 000) use seven separate proteins with five different colors.
• For use on SDS-PAGE

General description

Rainbow Molecular Weight Markers have been enhanced to enable faster and simpler identification of proteins on SDS-polyacrylamide gels. The ready-to-load markers provide sharper, more intense bands on gels and blots and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colors of the markers allow easier confirmation of transfer to blotting membranes and orientation.

Rainbow Molecular Weight Markers comprise a mixture of individually colored proteins that are combined to produce bands of equal color intensity. The approximate protein concentration is 1 to 2 mg/mL, depending on the product. Each 250 μl pack size is sufficient for use with 50 minigels (10 x 8 cm) or 25 large gels (20 x 20 cm).

Legal Information

Amersham is a trademark of GE Healthcare

ECL is a trademark of GE Healthcare

Rainbow is a trademark of GE Healthcare

Safety & Documentation

Safety Information

Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Same Products - New Packaging
Protocols & Articles

Articles

GE Western Blotting Introduction

Introduction, Extracted from Western Blotting Principles and Methods, GE Healthcare Life Science, 2011
Keywords: Catalytic combustion detector, Detection methods, Electrophoresis, Gel electrophoresis, Gene expression, Immobilization, Oxidations, Purification, Sample preparations, Separation, Western blot

Protocols

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

Refer to GE Healthcare Western Blotting Handbook (28-9998-97) for further information on optimization of antibody concentration for Western blotting.
Keywords: Tagged proteins, Western blot

Detection of GST-tagged Proteins with Western Blot

Expression and purification of GST-tagged proteins can be monitored by Western blot analysis, using Amersham ECL, Amersham ECL Prime, or Amersham ECL Select detection systems to enhance sensitivity.
Keywords: Electrophoresis, Gene expression, PAGE, Purification, Separation, Tagged proteins, Western blot

Troubleshooting of Detection Methods of GST-tagged Proteins

The troubleshooting guide below addresses problems common to the majority of detection methods as well as problems specific to a particular method. In the latter case, the relevant method is indicate...
Keywords: Buffers, Cell disruption, Detection methods, Electroblotting, Gene expression, Purification, Tagged proteins, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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