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GL0010 Sigma-Aldrich

Golgi Isolation Kit

sufficient for 50 g (tissue)

  •  NACRES NA.32

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Description

General description

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).

Application

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

Analysis Note

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

Kit component also available separately

Description

Product #

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Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL SDS P8340
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Organelle Isolation | BioFiles Volume 6, Number 5 — Centrifugation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, a...
Mark Frei
BioFiles v6 n5, 22–25
Keywords: Capture ELISA, Cell culture, Centrifugation, Diffusion, Direct immunofluorescence, Dot blot, Enzyme-linked immunosorbent assay, Flow cytometry, Immunoassay, Immunocytochemistry, Immunoelectrophoresis, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Indirect ELISA, Individual protein Immunoprecipitation, Microarray Analysis, Microscopy, Radioimmunoassay, Scanning electron microscopy, Western blot

Peer-Reviewed Papers
15

References

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