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  • GW10896A - Anti-EED antibody produced in chicken

GW10896A Sigma-Aldrich

Anti-EED antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Embryonic ectoderm development protein homolog, Anti-HEED

  •  NACRES NA.41



Related Categories A to L, Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Most Studied Epigenetic Genes,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   rat, mouse, human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-10896A
NCBI accession no.   NP_003788.2
UniProt accession no.   O00149
Gene Information   human ... eed(8726)



Immunogen Sequence: GI # 24041020, sequence 41-168

Recombinant embryonic ectoderm development isoform a


Anti-EED antibody produced in chicken is suitable for western blotting at a working dilution of 1:500 and for cell staining at a working dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The gene EED (embryonic ectoderm development) encodes a protein belonging to the Polycomb-group (PcG) family. These proteins are multimeric complexes that maintain the transcriptional repressive state of genes through successive cell generation. EED protein interacts with histone deacetylase (HDAC) proteins and mediates transcriptional repression. It forms a complex with the PRC2 histone methyltransferase complex along with EZH2 (enhancer of zeste 2) and SUZ12. This complex mediates the silencing of HOX gene expression during embryonic development. The complex plays a key role in regulating proliferation and embryogenesis. The PRC2/EED-EZH2 complex methylates ′Lys-9′ and ′Lys-27′ of histone H3 and mediates transcriptional silencing of the target genes. This complex serves as a recruiting platform for DNA methyltransferases.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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