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GW20021F Sigma-Aldrich

Anti-Fibronectin antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Cold-insoluble globulin, Anti-FN



Related Categories A to L, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Extracellular Matrix,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   indirect ELISA: suitable
  western blot: suitable
species reactivity   rat, mouse, human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-20021F
concentration   1.0-1.2 mg/mL
NCBI accession no.   CAA26536.1
UniProt accession no.   P02751
Gene Information   human ... FN1(2335)


General description

The gene FN1 (Fibronectin) is mapped to human chromosome 2q34. It is present as a soluble dimeric form in plasma. At the cell surface and in the extracellular matrix, it is present as a dimeric or multimeric form.


Fibronectin native protein.

Immunogen Sequence: GI # 31397, sequence 1-2328


Anti-Fibronectin antibody produced in chicken is suitable for western blotting at a working dilution of 1:500 and for cell staining at a working dilution of 1:200. It is also suitable for indirect ELISA.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Fibronectin (FN1) is a glycoprotein that is involved in cell adhesion and migration processes, such as embryogenesis, wound healing, blood coagulation, host defense, and metastasis. It binds to cell surfaces and compounds, such as collagen, fibrin, heparin, fibulin, integrins, cell adhesive mac-2 binding protein (M2BP), cartilage oligomeric matrix protein (COMP), DNA, and actin. Fibronectin interaction with FLRG (Follistatin-related gene protein) and follistatin is important for human hematopoietic cell adhesiveness. It is associated with glomerulopathy with fibronectin deposits, an autosomal dominant disease. Polymorphism in gene is linked with increased risk of knee osteoarthritis. Human pathogens, Staphylococcus aureus and Streptococcus pyogenes, target fibronectin for adhesion to and invasion of host cells. Pigment epithelium-derived factor (PEDF) down-regulates fibronectin to suppress breast cancer metastasis.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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