• USA Home
  • GW20073F - Anti-Complement C3 antibody produced in chicken

EMAIL THIS PAGE TO A FRIEND
GW20073F Sigma-Aldrich

Anti-Complement C3 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Purchase

Properties

Related Categories A to L, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   indirect ELISA: suitable
  western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-20073F
NCBI accession no.   NP_000055.1
UniProt accession no.   P01024
Gene Information   human ... C3(718)

Description

General description

Chicken polyclonal anti-Complement C3 antibody reacts with human complement C3.

Complement C3 plays a central role in the activation of the complement system. It’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability, and causes histamine release from mast cells and basophilic leukocytes.

The complement factor 3 (C3) gene, with 41 exons spanning 41kb on genomic DNA, is mapped to human chromosome 19p13.3. It is the third component of complement and is characterized with α and β chains. It is one of the most abundant complement protein in the serum.

Immunogen

Complement C3 native protein.

Immunogen Sequence: GI # 4557385, sequence 1-1663

Application

Anti-complement C3 antibody produced in chicken has been used in immunoblot analysis.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Chicken polyclonal anti-Complement C3 antibody is used to tag complement C3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA and western blotting. It is used as a probe to determine the presence and roles of complement C3 as a component of the complement system.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Complement factor 3 (C3) plays an essential role in the activation of the complement system. C3-convertase (C4b2a) cleaves C3 complement into C3a and C3b. C3b can bind covalently, through its reactive thioester, to cell surface carbohydrates or immune complexes and involve in the self-activation loop of complement activation via the alternate pathway. In addition, C3b also interacts with C3 convertases (C4b2a or C3bBb) to form the C5 convertase, which cleaves complement factor 5 into C5a and C5b. Derived from proteolytic degradation of complement C3, C3a (anaphylatoxin) facilitates the recruitment of inflammatory cells. C3a stimulates smooth muscle contraction, enhances vascular permeability and causes histamine release from mast cells and basophilic leukocytes. Mutation in C3 gene leads to the development of age-related macular degeneration (AMD).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?