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GW20081F Sigma-Aldrich

Anti-Histone H1,1 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Histones,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human, rat, mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-20081F
NCBI accession no.   P02253
UniProt accession no.   P02253
Gene Information   human ... HIST1H1A(3024)

Description

Immunogen

Histone H1.1 native protein.

Immunogen Sequence: GI # 121903, sequence 1-104

Application

Anti-Histone H1,1 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Histone H1.1 is a protein encoded by the HIST1H1A gene in humans and the gene is expressed in testis and thymus, but not in other human tissues. It is located on chromosome 6(6p21-p22) containing 55 histone genes. ist1 on mouse chromosome 13 contains 51 histone genes. There are 2 different human H1 histones coding genes that are differentiated mainly due to the N- and C-terminal H1 domains, whereas the central part of the protein is highly conserved. Histone H1 is a fundamental chromosomal protein and is associated with the maintenance of higher-ordered chromatin organization. It functions in the compaction of chromatin into higher order structures and its modulation is an important step in the potentiation/depotentiation of chromatin structure for transcription. H1 binds less tightly than other histones to DNA in chromatin and can readily exchange in living cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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