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GW20091F Sigma-Aldrich

Anti-Luciferase antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Luciferin 4-Monooxygenase

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, IgY Antibodies, LRR-LZ,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   firefly
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-20091F
NCBI accession no.   P08659
UniProt accession no.   P08659

Description

Immunogen

Immunogen Sequence: GI # 126501, sequence 1-550

Recombinant luciferin 4-monooxygenase (Luciferase)

Application

Anti-Luciferase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Luciferase is a member of the class of oxidative enzymes essential for bioluminescence. The term is originated from Lucifer; one example is firefly luciferase from the firefly Photinus pyralis. Its unique properties have spawned a number of novel applications of this protein. Luciferase has a number of applications such as a reporter gene, as a model for the study of protein import into peroxisomes and as a component of a heterologous gene expression system. Its structure reveals a new protein fold, which may act as a representative of a growing family of homologous enzymes.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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