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GW21052 Sigma-Aldrich

Anti-CKLF antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Chemokine-like factor, isoform b

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, CI-CN, IgY Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-21052
NCBI accession no.   NP_057410.1
UniProt accession no.   Q5BJH6
Gene Information   human ... CKLF(51192)

Description

Immunogen

Immunogen Sequence: GI # 7705933, sequence 27-67

Recombinant chemokine-like factor isoform c

Application

Anti-CKLF antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Chemokine-like factor is a protein encoded by the CKLF gene in humans. It is the first member of the CKLF-like MARVEL transmembrane domain containing member (CMTM) family and its expression is differentially regulated in myoblasts and myotubes. CKLF has a major role in the recruitment and activation of leucocytes that plays an important role in the pathogenesis of allergic diseases. It can induce proliferation and inhibit apoptosis of human vascular smooth muscle cell by down-regulating Bax and Caspase-3 and by the up-regulation of Bcl-2 expression. CKLF1 may play an important role in inflammation and regeneration of skeletal muscle. It may also play an important role in the development of atopic dermatitis, in the process of immune and inflammatory responses of atherogenesis. CKLF1 is a useful marker for regenerating muscle fibers in inflammatory myopathies.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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