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GW21183 Sigma-Aldrich

Anti-BRCA1 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Breast and ovarian cancer susceptibility protein splice variant

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Properties

Related Categories A to L, AA - CA, Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-21183
NCBI accession no.   NP_009225.1
UniProt accession no.   O15129
Gene Information   human ... BRCA1(672)

Description

Immunogen

Immunogen Sequence: GI # 6552299, sequence 1395-1454

Recombinant breast cancer 1, early onset isoform 1

Application

Anti-BRCA1 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

BRCA1 is a human tumor suppressor gene. Its main function is to maintain genomic stability through the assembly of multiple protein complexes involved in DNA repair, cell-cycle arrest and transcriptional regulation. BRCA1 is associated with breast and ovarian tumor suppression. Mutations in BRCA1 is involved in the pathogenesis of breast and ovarian cancer. BRCA1 promoter methylation is associated with better response to platinum-taxane-based therapy in epithelial ovarian cancer (EOC). BRCA1 mutation may drive the formation of tumors in estrogen-regulated tissues.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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