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GW21187 Sigma-Aldrich

Anti-E2F2 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Transcription factor E2F2

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Properties

conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human, mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-21187
NCBI accession no.   NP_004082.1
UniProt accession no.   Q14209
Gene Information   human ... E2F2(1870)

Description

Immunogen

Immunogen Sequence: GI # 4758226, sequence 348-437

Recombinant E2F transcription factor 2

Application

Anti-E2F2 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Transcription factor E2F2 is a protein encoded by the E2F2 gene in humans. It is associated with the regulation of cell-cycle and blood pressure. E2F2 is a transcription factor that helps regulate the expression of a number of genes that are important in cell proliferation. The cell cycle progression is dependent on the transcriptional activity of E2F2 and are associated with pocket proteins that binds to DNA as a heterodimer of two distinct proteins. E2F-2 is functionally and immunologically associated with one of these two distinct proteins.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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