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GW21319 Sigma-Aldrich

Anti-RGS13 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Regulator of G-protein signaling 13

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IgY Antibodies, M to Z, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human, rat, mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-21319
NCBI accession no.   NP_002918.1
UniProt accession no.   O14921
Gene Information   human ... RGS13(6003)

Description

General description

Regulator of G-protein signaling 13 is a protein encoded by the RGS13 gene in humans. It is a member of R4 subfamily of RGS (Regualtors of G Protein Signaling) proteins and is a GTPase-activating protein for Gα(i) and Gα(o). RGS13 helps in suppressing IgE-antigen-induced MC degranulation and anaphylaxis. It is the smallest member of the RGS family and is abundantly found in human tonsil, followed by thymus, lung, lymph node and spleen.

Immunogen

Immunogen Sequence: GI # 4506511, sequence 42-99

Recombinant regulator of G-protein signalling 13

Application

Anti-RGS13 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Regulator of G-protein signaling 13 (RGS13) expression is induced by immune receptor and chemokine receptor stimulation. It may help in regulating Gα(i)-, Gα(q)- and Gα(s)-coupled signaling cascades. RGS13 inhibits the assembly of immune receptor-induced signalosomes in mast cells and its abnormal expression or function may contribute to disorders of amplified mast cell activity (such as idiopathic anaphylaxis). RGS13, along with RGS1, acts together to regulate chemokine receptor signaling in human germinal center B lymphocytes and contribute significantly to the rapid desensitization of the signaling pathway. It restricts certain GPCR-mediated biological responses of human mast cells. RGS13 inhibits cyclic AMP-dependent protein kinase (PKA)-induced gene expression through interactions with the transcription factor cAMP-response element-binding (CREB) protein.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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