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GW22280A Sigma-Aldrich

Anti-KLHL12 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, IgY Antibodies, KL-KY,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22280A
NCBI accession no.   NP_067646.1
UniProt accession no.   Q53G59
Gene Information   human ... KLHL12(59349)

Description

General description

Kelch-like protein 12 is a novel human kelch protein localized to a ring canal structure. It consists of N-terminal POZ (poxviruses and zinc fingers) and intervening region (IVR) domains. It plays an important role in the ring-like structure activity during oocyte development.

Immunogen

Immunogen Sequence: GI # 11056006, sequence 205-404

Recombinant kelch-like 12

Application

Anti-KLHL12 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Kelch-like protein 12 is a protein encoded by the KLHL12 gene in humans. It is a Bric à Brac (BTB)-Kelch protein and specifically binds to the polymorphic region of the D4 receptor through its Kelch domain. KLHL12 also interacts with Cullin3 and functions as an adaptor to target the D4 receptor to an E3 ubiquitin ligase complex. Its overexpression strongly promotes ubiquitination of the D4 receptor. KLHL12 is essential for the development of the ring-like structure, which is independent of the ability to bind actin. It interacts with both immature, ER-associated and mature, plasma membrane-associated D4 receptors, β-arrestin2, and also promotes ubiquitination of both receptor subpools.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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