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GW22306F Sigma-Aldrich

Anti-QPRT antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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Properties

species reactivity   human
application(s)   western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... QPRT(23475)
biological source   chicken
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22306F
conjugate   unconjugated
NCBI accession no.   NP_055113.2
UniProt accession no.   Q15274

Description

Immunogen

Immunogen Sequence: GI # 45269149, sequence 123-237

Recombinant quinolinate phosphoribosyltransferase

General description

QPRT (quinolinate phosphoribosyltransferase) is an essential enzyme of quinolinate catabolism, and has a molecular weight of 34kDa. It is found in both the cytoplasm and nucleus, and is expressed in central nervous system cells, platelets, erythrocytes and liver. It belongs to the type II phosphoribosyltransferase family. Its active site is situated at an α/β open sandwich region.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Application

Anti-QPRT antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Mammalian quinolinate phosphoribosyltransferase (QPRTase) is a key enzyme in catabolism of quinolinate. It is an intermediate in the tryptophan-nicotinamide adenine dinucleotide (NAD) pathway. Quinolinate acts as a potent endogenous exitotoxin to neurons and an elevation in its levels in the brain is linked to the pathogenesis of neurodegenerative disorders. QPRT acts at the junction of two different pathways, de novo NAD+ biosynthesis and the kynurenine pathway of tryptophan degradation. It is an important enzyme in terms of its biological impact and its potential as a therapeutic target.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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