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  • GW22331B - Anti-KAT (ab2) antibody produced in chicken

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GW22331B Sigma-Aldrich

Anti-KAT (ab2) antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, IgY Antibodies, K-KH,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   mouse, human, rat
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22331B
NCBI accession no.   NP_005877.1
UniProt accession no.   Q59G59
Gene Information   human ... KAT(10300)

Description

General description

KATNB1 (katanin p80 subunit B 1) forms the regulatory component of katanin protein, which also contains the catalytic p60 subunit. This protein is composed of procon80 domain at its C-terminal, and a WD40 domain at its N-terminal. This gene is highly conserved in humans, spans ~1.6kb, and is composed of four exons. It codes for a protein of 115 amino acids, with a molecular weight of 12.5kDa. It has a wide range of tissue expression such as, thymus, heart, lung, liver, kidney, spleen, colon, skeletal muscle and placenta.

Immunogen

Immunogen Sequence: GI # 5031817, sequence 301-511

Recombinant katanin p80 subunit B 1

Application

Anti-KAT (ab2) antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The KAT (thiosulfate sulfurtransferase (rhodanese)-like domain containing 1) protein is found around the nuclear membranes and is expressed in several breast, colon and lung carcinoma cell lines, but not in normal breast epithelial cell lines. It is also detected in invasive ductal carcinoma, but not in adjacent tissues and has a role in tumorigenesis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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