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GW22398F Sigma-Aldrich

Anti-GCA antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, G-GC, IgY Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22398F
NCBI accession no.   P28676
UniProt accession no.   P28676
Gene Information   human ... GCA(25801)

Description

Immunogen

Immunogen Sequence: GI # 1170014, sequence 1-217

Recombinant grancalcin

Application

Anti-GCA antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Grancalcin is a protein encoded by the GCA gene in humans and is a member of the EF-hand family of calcium-binding proteins called PEF proteins. It is a EF-hand Ca2+-binding protein (28kDa) particularly abundant in neutrophils and monocytes, with relatively small amounts in lymphocytes. Grancalcin consists of four EF-hand calcium-binding motifs and exhibits strong homology to sorcin, one of two proteins overexpressed in multidrug-resistant cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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