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GW22454A Sigma-Aldrich

Anti-UME6 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, IgY Antibodies, M to Z, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22454A
NCBI accession no.   NP_010493.1
UniProt accession no.   P39001
Gene Information   yeast ... UME6(851788)

Description

Immunogen

Immunogen Sequence: GI # 6320413, sequence 339-808

Recombinant key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rp

Application

Anti-UME6 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

UME6 gene of Saccharomyces cerevisiae is identified as a mitotic repressor of early meiosis-specific gene expression and encodes a Zn2Cys6 DNA-binding protein. It binds to URS1, a promoter element required for both mitotic repression and meiotic induction of early meiotic genes. UME6 is part of a developmental switch that controls both vegetative repression and meiotic induction of meiosis-specific genes. It plays an important role in spore germination and the first few mitotic cell divisions. UME6 gene also has both negative and positive roles in regulating phospholipid biosynthesis.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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