• USA Home
  • GW22527F - Anti-CAPG antibody produced in chicken

EMAIL THIS PAGE TO A FRIEND
GW22527F Sigma-Aldrich

Anti-CAPG antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

Purchase

Properties

Related Categories A to L, Alphabetical Index, Antibodies, CAM-CB, IgY Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human, mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22527F
NCBI accession no.   NP_001738.1
UniProt accession no.   P40121
Gene Information   human ... CAPG(822)

Description

General description

CAPG (capping protein, gelsolin-like) is an actin-binding protein, which is expressed ubiquitously. It belongs to the Ca2+- and polyphosphoinositide-regulated villin/gelsolin superfamily, and caps the barbed end of the growing actin microfilament.

Immunogen

Immunogen Sequence: GI # 4502561, sequence 1-348

Recombinant capping protein (actin filament), gelsolin-like

Application

Anti-CAPG antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Macrophage-capping protein (CAPG) plays a critical role in regulating the migration ability of cells. It is a Ca2+-sensitive protein, which reversibly blocks the barbed ends of actin filaments, but does not sever preformed actin filaments. CAPG helps in promoting tumor cell invasion, but not cell proliferation. It is involved in the process of metastasis by promoting the invasiveness of tumor cells. It can act as a biomarker in risk-stratification therapy for gastric cancer and as a therapeutic target in Hepatocellular carcinoma (HCC). CAPG is also identified as a novel candidate biomarker to predict response to gemcitabine treatment and survival in cholangiocarcinoma as well as lung adenocarcinoma.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?