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GW22538F Sigma-Aldrich

Anti-GRB2 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   rat, human, mouse
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22538F
NCBI accession no.   NP_002077.1
UniProt accession no.   P62993
Gene Information   human ... GRB2(2885)

Description

General description

GRB2 (growth factor receptor-bound protein 2) is an adaptor protein, which is expressed ubiquitously. It is composed of two SH3 domains and one SH2 domain. The SH3 domains are present at the N- and C-termini, and are intervened by SH2 domain. It exists as a heterodimeric protein.

Immunogen

Immunogen Sequence: GI # 4504111, sequence 1-217

Recombinant growth factor receptor-bound protein 2 isoform 1

Application

Anti-GRB2 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Growth factor receptor-bound protein 2 (GRB2) is involved in signal transduction/cell communication. GRB2 controls fibroblast growth factor receptor 2 (FGFR2) signaling by regulating receptor kinase and SH2 domain-containing protein tyrosine phosphatase 2 (Shp2) phosphatase activities in the absence of extracellular stimulation. The protein is involved in the activation of the Ras signaling pathway. It helps in the regulation of Receptor tyrosine kinases (RTKs) signaling. GRB2 is associated with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) through its SH2 domain. It plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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