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GW22594 Sigma-Aldrich

Anti-DUSP12 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, DQ-DZ, IgY Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
NCBI accession no.   NP_009171.1
UniProt accession no.   Q5VXA8
Gene Information   human ... DUSP12(11266)

Description

General description

DUSP12 (dual specificity phosphatase) gene is localized to human chromosome 1q21-q22. Its C-terminal contains a non-catalytic domain, which is rich in cysteine and acts as a zinc finger domain. It is an atypical DUSP member, which is recognized as a phosphatase with pro-survival attributes. It is evolutionarily conserved. Its DUSP catalytic domain is present at its N-terminal.

Immunogen

Immunogen Sequence: GI # 6005956, sequence 1-340

Recombinant dual specificity phosphatase 12

Application

Anti-DUSP12 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Dual specificity protein phosphatase 12 (DSUP12) is a novel cell survival phosphatase. DUSP12 is an evolutionary conserved phosphatase containing a unique zinc-binding domain. Its expression affects cell cycle progression. Overexpression of the gene increases cell motility and resistance to apoptosis. Overexpression also causes a significant increase in polyploidy and in the G 2/M cell population, with a subsequent decrease in the G 0/G 1 population. It promotes increased expression of the c-met proto-oncogene and collagen, and laminin receptor intergrin α 1 (itgα1), which is implicated in metastasis. DUSP12 is oncologically relevant and could be used therapeutically for targeted oncogenes. The enzyme plays a role in cell survival and ribosome biogenesis and protects cells from oxidative stress.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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