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  • GW22609 - Anti-CDKN1B antibody produced in chicken

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GW22609 Sigma-Aldrich

Anti-CDKN1B antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, CDH-CDY, IgY Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   chicken
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22609
NCBI accession no.   NP_989587.1
UniProt accession no.   Q8JIV2
Gene Information   chicken ... CDKN1B(374106)

Description

Immunogen

Immunogen Sequence: GI # 45383622, sequence 1-198

Recombinant cyclin-dependent kinase inhibitor 1B (p27, Kip1) [Gallus gallus]

Application

Anti-CDKN1B antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Cyclin-dependent kinase inhibitor 1B (p27Kip1) is an enzyme inhibitor that, in humans, is encoded by the CDKN1B gene and belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. It acts as a multifunctional protein to control critical steps of neuronal migration through activities that go far beyond cell cycle regulation. Mutation in CDKN1B gene causes an autosomal dominant disorder called multiple endocrine neoplasia type 4 (MEN4). The cell cycle inhibitor CDKN1B is involved in ERK1/2-mediated MMP-9 expression through activation of the NF-κB binding motif, which leads to the migration and invasion of bladder cancer cells induced by IL-7.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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