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GW22677 Sigma-Aldrich

Anti-DLG7 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, DE-DL, IgY Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
NCBI accession no.   NP_055565.2
UniProt accession no.   Q15398
Gene Information   human ... DLGAP5(9787)

Description

General description

DLGAP5 (discs large homolog-associated protein 5) or DLG7, acts as a mitotic phosphoprotein substrate for Aurora-A. It was recognized 10 years ago, and is a Ser/Thr kinase, which is modulated by cell cycle. It contains a highly conserved domain called guanylate-kinase-associated protein (GKAP) guanylate-kinase-associated protein (GKAP). It is localized to cytoplasm, and is highly expressed in fetal liver. In adults, its predominant expression is in bone marrow, colon, testis and placenta.

Immunogen

Immunogen Sequence: GI # 21361645, sequence 1-300

Recombinant discs large homolog 7

Application

Anti-DLG7 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

The expression of discs large-associated protein 5 (DAP-5, also known as DLG7) is hypoxia-controlled and holds prognostic potential in high-risk prostate cancer (CaP). It can act as a novel biomarker for prostate cancer (CaP). Expression of DLGAP5 is regulated by methylation and the up-regulation contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation. It is useful for non-invasive test for early detection of bladder cancer and bilharzial bladder cancer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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