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GW22678 Sigma-Aldrich

Anti-ERB antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Transcription Factors,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   human, mouse, rat
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22678
NCBI accession no.   NP_001428.1
UniProt accession no.   Q59F41
Gene Information   human ... ESR2(2100)

Description

General description

Estrogen receptor β (ERB) belongs to a family of nuclear receptors, which function as regulators of homeostasis. There are two types of ER- ERα and ERβ, and ERs form the class I of nuclear receptors. They are normally sequestered as multi-protein complex in the cytoplasm, until activated by their ligands. It contains a DNA-binding domain, a hinge domain, and AF1 domain, and a ligand-binding domain. It has a high level of expression in normal breast cells.

Immunogen

Immunogen Sequence: GI # 10835013, sequence 1-150

Recombinant estrogen receptor β isoform 1

Application

Anti-ERB antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Biochem/physiol Actions

Estrogen receptor β (ERB) functions as a transcription factor, and modulates the transcription of target genes, which are components of cell cycle including cyclins and proto-oncogenes. It activates growth-inhibitory genes and thus, functions in suppressing cell proliferation. It negates the activation of anti-apoptotic genes by ERα, via 17β-estradiol. It is inactivated in breast cancer (BC), which marks an important stage in the progression of BC, in an estrogen-dependent manner. The presence of this gene is BC has prognostic value for tamoxifen-treated patients, and might also have potential as a therapeutic target. Mitochondrial ERβ2 has anti-apoptotic functions, and thus predicts poor prognosis in serous ovarian cancer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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