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GW22683 Sigma-Aldrich

Anti-ENO1 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies to Transcription Factors,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   mouse, human, rat
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22683
NCBI accession no.   NP_001419.1
UniProt accession no.   P06733
Gene Information   human ... ENO1(2023)

Description

General description

Enolase 1 (ENO1 or ENOα) is a type of enolase enzyme, which in mammals, has three different isoforms namely, α-, β- and γ-enolase. It is an essential cytoplasmic glycolytic enzyme, and has a molecular weight of 48kDa. An alternatively spliced form resides in the nucleus, which has a molecular weight of 37kDa.

Immunogen

Immunogen Sequence: GI # 4503571, sequence 161-320

Recombinant enolase 1

Application

Anti-ENO1 antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Enolase 1(α-enolase) is a homodimer composed of 2 α subunits. ENO1 is involved in tumor progression and prognosis of human glioma. Overexpression of ENO1 is associated with progression of glioma while its knockdown leads to suppressed cell growth, migration and invasion progression by inactivating the PI3K/Akt pathway in glioma cells. The lower expression of ENO1 decreases the response to hypoxia and enhances the sensitivity of the cells to radiation therapy. It may act as a drug target for the treatment of breast cancer.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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