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GW22776 Sigma-Aldrich

Anti-AKT2 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories A to L, AE-AK, AKT Antibodies, AKT2 Antibodies, Alphabetical Index,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   indirect ELISA: suitable
  western blot: suitable
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22776
NCBI accession no.   NP_001617.1
UniProt accession no.   P31751
Gene Information   human ... AKT2(208)

Description

Immunogen

Immunogen Sequence: GI # 4502023, sequence 108-124

Synthetic peptide sequence of V-akt murine thymoma viral oncogene homolog 2.

Application

Anti-AKT2 antibody produced in chicken is suitable for indirect ELISA and western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

RAC-β serine/threonine-protein kinase is an enzyme encoded by the AKT2 gene in humans and is mapped to chromosome region 19q13.1-q13.2. It belongs to a distinct subfamily of protein-serine/threonine kinases containing Src homology 2-like domains. Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas. The protein kinase is thought to be a key mediator of the insulin signal transduction process and plays a role in glucose metabolism. It also mediates the development or maintenance of proper adipose tissue and islet mass. It is an important protein in PI3K signaling pathway and is overexpressed in a variety of malignant tumors. AKT2 may be a useful molecular marker for predicting the prognosis of meningioma. It plays a novel role in neuroblastoma tumorigenesis and may be important for developing novel therapeutics for the treatment of clinically aggressive neuroblastoma.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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