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GW22831 Sigma-Aldrich

Anti-ADAMTS-12 antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

  •  NACRES NA.41

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Properties

Related Categories A to L, AD-AD, Alphabetical Index, Antibodies, IgY Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   mouse, human, rat
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
mfr. no.   Genway, 15-288-22831
NCBI accession no.   NP_112217.1
UniProt accession no.   P58397
Gene Information   human ... ADAMTS12(81792)

Description

General description

A disintegrin and metalloproteinase with thrombospondin motifs 12 is an enzyme encoded by the ADAMTS12 gene in humans. It belongs to the family of metalloproteinases that helps in mediating a communication between specific cell types and plays a key role in the regulation of normal tissue development, remodeling and degradation.

Immunogen

Immunogen Sequence: GI # 13569928, sequence 297-446

Recombinant A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 12

Application

Anti-ADAMTS-12 antibody produced in chicken is suitbale for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

ADAMTS12 is a degradative enzyme that interacts with the degradable fragments of cartilage oligomeric matrix protein, which is a prominent non-collagenous matrix component in articular cartilage. The protein exhibits a propeptide and metalloproteinase-like, disintegrin-like cysteine-rich domains. It may play a crutial role in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion. ADAMTS12 is found in cartilage, synovial fluid and serum of arthritic patients and may play an important role in the pathogenesis of arthritis. It may be a susceptibile gene for rheumatoid arthritis development. The genetic variations in ADAMTS12 is found to influence the risk of schizophrenia.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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