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H0135 Sigma-Aldrich

Hydrocortisone

γ-irradiated, powder, BioXtra, suitable for cell culture

Synonym: 11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M

  • CAS Number 50-23-7

  • Empirical Formula (Hill Notation) C21H30O5

  • Molecular Weight 362.46

  •  Beilstein/REAXYS Number 1354819

  •  EC Number 200-020-1

  •  MDL number MFCD00011654

  •  PubChem Substance ID 24895394

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Corticosteroid, Cytokines, Growth Factors and Hormones,
Quality Level   200
biological source   synthetic (organic)
sterility   γ-irradiated
product line   BioXtra
assay   ≥80% (HPLC)
form   powder
application(s)   cell culture | mammalian: suitable
mp   211-214 °C (lit.)
solubility   ethanol: 1 mg/mL, clear
shipped in   ambient
storage temp.   room temp
SMILES string   [H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
InChI   1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChI key   JYGXADMDTFJGBT-VWUMJDOOSA-N
Gene Information   human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)

Description

General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Application

Hydrocortisone is used for the following applications:
• Used in myogenic induction and identification
• Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
• Used in adipogenic differentiation (one of the components in adipogenic medium)
• Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
• The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
• Used in differentiation assays of melanoma spheroid cells

Biochem/physiol Actions

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
GM8925000

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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H6909 Hydrocortisone solution, 50 μM, sterile-filtered, BioXtra, suitable for cell culture

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